The Consequences of Chronic Unpredictable Stress Exposure to Spatial Memory of Adolescent Mice (Mus musculus albinus).

Abstract

Adolescence is a sensitive period when neurogenesis rate is increases. Chronic stress that occurs during adolescence can cause a long-term effects on adult behavior and morphology of hippocampus. Light-dark transition test showed that CUS successfully produced depressed adolescent mice. Y-alternation test revealed that short-term effect of CUS improved spatial memory in mice, resembled the capability of non-stressed adult while cell counting performed with HE staining did not showed a significant effect on proliferation, cell maturing, and cell survival in DG. Complexity of dendrites in DG and pyramidal neurons of CA1 performed using Golgi-Cox staining. Apical dendrites of DG did not showed a significant effect of CUS on dendritic complexity of DG. In contrast, both of apical and basal dendrites of CA1 showed a significant short-term effect of CUS, seen from the number of intersections. Maximum dendritic span and total dendritic length did not show a significant differences. It is remarkable that both short and long-term effect of CUS is changes the complexity of CA1 pyramidal neuron and transciently increases spatial memory on adolescent mice.