POTENSI Trichoderma sp. dan Gliocladium sp. SEBAGAI JAMUR ANTAGONIS TERHADAP Cylindrocladium sp. PENYEBAB PENYAKIT LODOH PADA PERSEMAIAN SECARA IN-VITRO

Abstract

Damping-off is one of the disease that commonly attack plantation in nursery which is caused by pathogenic fungi Cylindrocladium sp. Biological control using antagonic fongi Trichoderma sp. and Gliocladium sp. is an alternative that is expected to reduce pollution risk by minimizing disturbance of biological balance. This study had objective to identify potency of Trichoderma sp. and Gliacladium sp. as biological control for Cylindrocladium sp. pathogen that causes damping-off disease in nursery of forest plantation through in-vitro. Trial was carried out in petridishes with method of dual culture using FDA media. Split plot design with five treatments was applied. Treatmens consisted of A ~ one colony of Cylindrocladium sp. pathogenicfongi with one colony of Trichoderma sp. antagonicfongi (CT 1: 1); B ~ one colony of Cylindrocladium sp. pathogenic fungi with two colony of Trichoderma sp. antagonic fungi (CT 1 : 2); C ~ one colony of Cylindrocladium sp. pathogenic fungi with one colony of Gliocladium sp. antagonic fungi (CG 1 : 1); D ~ one colony of Cylindrocladium sp. pathogenic fungi with two colony of Gliocladium sp. antagonic fongi (CG I : 2). Each treatmen had five replications. The parameters measured were the diameter growth of Cylindrocladium sp. and inhibiting percentage of antagonic fongi to Cylindrocladium sp. The result showed that treatment A and B on the sixth day inhibited the growth of pathogenic fongi by 24,2% and 22,4% respectively. Treatment C dan D on the sixth day inhibited growth of pathogenic fongi by 19,3% and 15,2% respectively. The inhibiting was caused by antibiotic and lysis activity, growth competition by Trichoderma sp and Gliocladium sp. to Cylindrocladiium sp.