CHARACTERS OF SPECIES PLEUROTOID: MORPHOLOGICAL, PHYSIOLOGICAL AND LIGNINOLYTIC STUDIES TO SUPPORT BIOINDUSTRY

Abstract

Morphological, physiological and ligninolytic characterization of pleurotoid species from Bogor district were studied. The method for isolation of pleurotoid was a tissue culture conducted, using modified MEA medium. The selected isolates were maintained and cultured on various media. To identify, the mycelium, fruiting-body, basidiospores, cystidia and other structures of the fungi were observed using a microscope stereo-monocular and scanning electron microscopy (SEM). The physiological aspects of the fungi were characterized based on the growth rate, oxidation.reaction, vegetative and reproductive phase, total biomass and biological efficiency. The growth rate of the fungi was observed on media PDA, MEA, MPA with different temperatures and pH. The oxi,dation reaction was conducted using AAG and AAT media. The ligninolitic activity of fungi was measured based on the biodegradation of Sengon (Paraserianthes falcataria) wood sawdust substrate by the fungi. More than 30 isolates of Pleurotoid were isolated from several places in Bogar. Six isolates of those isolates were able to fqrm fruiting bodies on media namely Pleurotus djamor EB9 and five isolates of Hohenbuehelia petaloides. Analysis cluster of isolates based on the morphological and physiological characterization showed that P. djamor EB9 was different from H. petaloides. Classification based on an ligninolytic character was different from classification based on morphological and physiological characters. P. djamor EB9 seemed to be separated from other isolates. This shows that there was different Jigninolytic character among the isolates. The best isolate for bia-pulping and bio-bleaching agent was P. djamor EB9 on vegetative phase.