Identification Polymorphisms of Inos Gene and Association with Body ResistanceTrait in Kampong Chicken

Abstract

INOS gene is one of the genes that play important factor in immunity related to resistence disease through non specific immune response in chicken. The aim of this study was to identify the polymorphism of iNOS gene in kampong chicken and it is association with resistance to the bacteria Salmonella pullorum. Genetic polymorphism of chicken was investigated using PCR RFLP method. There were three stages of identification: extraction of DNA genom, PCR amplification of iNOS gene (with size 449 bp), and RFLP method using restriction enzym (AluI). The data were analyzed include of genotype and allele frequencies, heterozygosity values and Hardy-Weinberg equilibrium (HW). The result showed that iNOSgene was showed polymorphic in kampung chickens population. It were found two alleles (T and C ), and three genotypes (TT, TC and CC). The chi square test revealed the locus of iNOS was deviated in Hardy Weinberg equilibrium. Association analysis indicated that CC genotype was signifcantly associated (P < 0.05) withdisease resistance than TC and TT genotypes in Kampung chicken, that‘s mean TC and TT genotype has hight disease resistance than CC. In conclusion the polymorphism of chicken iNOS gene could be potential to the candidate gene in breeding programe for increasing genetic resistance against S. pullorumin kampung chickens