Association Analysis of NRAMP1 Gene Related to Resistance Against Salmonella pullorum Infection in Kampung Chicken

Abstract

Natural Protein Resistance-Associated Makrofag 1 (NRAMP-1) gene plays a role in controlling disease resistance. Kampung Chicken is one of the local Indonesian chickens which have high diversity in term of productivity. Breeding programs to improve disease resistance through molecular selection is one of the efforts that have evolved to increase theproductivity of chicken. The present research was to study the association of apolymorphism of NRAMP-1 with resistance disease in Kampung chicken. The PCR-RFLP method was applied to analyze the association between the polymorphism of NRAMP1 with resistant disease againstSalmonella Pullorum. NRAMP-1 gene was genotyped in Kampung chickens using thePCR-RFLP method. The result showed three genotypes were identified of NRAMP-1 gene in Kampung chicken, namely TT, TC, and CC. The NRAMP-1 gene was polymorphic in all native chickens. The chi-square value of the Kampung chicken showed deviate in Hardy-Weinberg equilibrium. The TT and TC genotype revealed higher (P<0.05) mortality bacterium compare to CC genotype. In conclusion, polymorphism in chicken NRAMP-1 gene could be used as acandidate gene to increase resistance to disease in Kampung chicken.