Effect of Freezing on Bovine Sperm Morphology

Abstract

The evaluation of sperm morphology is an important component of the spermia-gramme and therefore of the andrological evaluation. Cryopreservation processes will reduce sperm motility, acrosome intact. viability as well as sperm morphology. This research aimed to study the effect of cryopreservation to bovine sperm morphology. Six bull consist of two Friesian Holstein (FH), two Simmental qnd two Limousin e belong to Lembang artificial insemination centre were used as a semen source. Semen was collected and processed according to artificial insemination center protocol . Primary sperm morphology evaluate by using bright-field microscopy of carbolfuchsin eosin stained dry-mount semen smears and secondary sperm morphology by phase contrast microscopy of wet-mount semen 'fixed' in isotonic formal-saline . Result demonstrated that Limousine had a lowerprimary and high secondary sperm abnormality (p<O.05) of raw semen compare to FH and Simmental bulls. Cryopre'servation processed increase primary sperm abnormality of Simmental bull and secondary sperm abnormality of FH bull. In total the increasing of sperm abnormal morphology during cryopreservation of three breed were between 1.14 to 1.31%. This research concluded that the cryopreservation processed in Artificial insemination center did not affected on the sperm morphology.