Determination of Extact Concentration in Tris Buffer of Frisian Holstein Chilled Semen

Abstract

This aim research was to investigate the best soy extract concentration on the quality of Frisian Holstein (FH) bulls chilled semen.Semen were collectedfrom 5 FH bulls belong to Lembang Artificial Insemination Centre using artificial vagina. Immediatly after colection the semen evaluate macro-and microscopycally. Semen samples with >75% progressive motility and contained <20% sperm with abnormal morphology were used in the experiment. Semen individually divided into 4 tubes and diluted with 2.5%, 3.75%, 5%, or 6.25% Tris soy extract (TSE), diluted semen than store at 5 °C. Sperm motility and viability were evaluate once a day. Theresults showed that sperm diluted in2.5% TSE was the highest sperm motility (30.25±8.45%) at 72 hours of storage (day 3) than in 3.75% (18.00±5.12%), 5% (7.17±2.32%) or 6.25% (4.45±1.02%) TSE. No differences wasfound on the viabiliy of sperm, between allfour TSE concentrations at 0 and 12 hours of storage. At 24 until 72 hour of storage, semen diluted with 3.5% TSE significantly higher than other concentration. This research conclude that sperm motility diluted in 2.5% soy extract better than other concentration, while 3.75% soy extract maintain sperm viability better than other concentration.