Indonesian Phalaenopsis amabilis Poliploidization Using Colcidcine

Abstract

The potted or cut Phalaenopsis sp. bas high economic value. The size, stalk length and Rower number, colour, shelf life and pest resistant determined the plant quality. For Indonesia new varieties development is important, since most of the Phalaenopsis hybrids in the market were introduced. On the other band orchid is suffering from uncertain future through over exploitation, habitat loss through human activities and impact or climate change. In nature endopolyploidy occours to cope with environment changes, such as increasing temperature, drought and bigh radiation caused by global warming. The first research was aimed to find new varieties or Indonesian Phalaenopsis amabillis from poliploidization using colchicine. The first experiment used flower, bud, and spike with 0, 50, 500, 1000 and 2000 mg l-1 in colchicine for 3 and 5 days arranged in Randomized Complete Block Design. The second experiments were in vitro experiments in half strength of Murahige-Skoog (1/2 MS) liquid media, using : (a) protocorms immened with 0, 05, 5, 25, 50 and 75 mg l-1 colchicine arranged in Randomized Complete Block Design, (b) plantlets immened with 0, 1000, 2000, 3000, 4000, and 5000 mg l-1 colchicine arranged in Randomized Complete Block Design. Both experiments produced polyploid plants