CTAB’S MODIFICATION: HIGH-QUALITY PLANT DNA EXTRACTION OF TOMATO FOR PCR WITH HEAT SHOCK TREATMENT

Abstract

DNA extraction is a crucial first step in PCR activities, especially for plants that contain secondary metabolites. Secondary metabolites may reduces the quality of the extraction and damages the DNA. The aim of this research was to get a modified of CTAB which can improve the quality of the extraction and storability of the DNA in tomatoes. This research used 6 local genotypes of tomato and primer RAPD (OPH 5). Each genotype was divided into two samples and treated with heat shock and without heat shock treatment. The heat shock treatment uses incubation at 950C for 5 minutes and saved at room temperature. PCR-RAPD activities has done every week for 8 weeks. Observation on electrophoresis genome shows that heat shock treatment results of DNA extraction is cleaner than without heat shock treatment. Heat shock treatment also increases the storability of DNA that can be saved at room temperature. RAPD results showed that heat shock treatment give a better consistency results than the amplification without heat shock treatment.