Suplementasi Sodium Dodecyl Sulphate dan Vitamin E terhadap Kualitas Semen Cair Ayam Lokal yang Dipreservasi pada Suhu 5 oC

Abstract

The objective of this experiment was to determine the effect of sodium dodecyl sulphate (SDS) and vitamin E supplementation to the lactate ringer-egg yolk (LREY) diluents on the pelung chicken semen preservation and to compare the semen quality of various local chicken breeds by in-vivo and in-vitro. This study was divided into several step; 1) SDS supplementation in the LREY diluent, 2) Vitamin E supplementation in the LREY diluent with best level of SDS, 3) The appeal test of local chickens semen quality with the best diluent in phase 2, 4) The appeal test of local chickens semen fertility and fertile period through artificial insemination in laying hens ISA Brown with the best diluent in phase 2. Step 1 to step 3 were design using a completely randomized repeated measurement with 4 replications, while step 4 was design using a completely randomized with 3 replications. Results of step 1 showed the sperm motility and viability in LREY added with 0.025% SDS (72.08±1.44% and 1.30±80.82%) was significantly higher (P<0.05) than 0% and 0.05% SDS at 24 hours of storage. No differences in sperm motility and viability between 0% and 0.05% SDS. The decrease of sperm motility and viability demonstrated that 0.025% SDS supplementation (4.17±2.89% and 4.65±1.63%) was significantly lower (P<0.05) than 0% and 0.05% SDS at 24 hours of storage. Results of step 2 showed sperm motility and viability in LREY with 0.025% SDS which supplemented with 2% of vitamin E (77.92±0.74% and 87.37±0.75%) was significantly higher (P<0.05) compared to 0%; 0.5%; 1% and 3% vitamin E at 12 hours of storage. The decrease of sperm motility and viability demonstrated 2% vitamin E supplementation (2.92±0.74% and 0.65±5.52%) was significantly lower than 0%; 0.5%; 1% and 3% vitamin E at 12 hours of storage. Results of step 3 showed chicken breeds affected on sperm motility and viability (P<0.05). During the storage period, the highest sperm motility and viability were demonstrated by pelung chicken. Sperm motility and viability of pelung chicken (72.50±1.12% and 81.94±1.12%) were higher than sentul chicken (68.33±1.05% and 80.95±1.06%), merawang chicken (65.83±1.54% and 76.83±1.26%) and kampung chicken (64.17±1.54% and 77.96±1.38%) at 24 hours storage. Results of step 4 showed chicken breeds affect on fertility (P<0.05), but did not affect the fertile period. Fertility of pelung chicken (62.10±2.76%) was higher than kampung chicken (49.47±3.68%) and merawang chicken (48.15±1.85%), but no difference between sentul chicken fertility (57.94 ± 4.83%) with others. The longest fertile period was demonstrated by pelung chicken (11.33±0.33 days), followed by sentul chicken (11.00±0.58 days), kampung chicken (10.33±0.88 days) and merawang chicken (10.00±0.58 days). In conclusion, SDS 0.025% and 2% vitamin E supplementation in LREY could maintain the sperm motility and viability during 72 hours of storage at 5 °C. Sperm motility and viability of pelung chicken were higher than sentul chicken, kampung chicken and merawang chicken during 72 hours of storage at 5 °C. The highest fertility was demonstrated by pelung chicken and the lowest by merawang chicken. Fertile period of four chicken breeds ranged from 10 to 11 days.