Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/77097
Title: KARAKTERISASI PROTEASE DARI ISOLAT BAKTERI ASAL TUMBUHAN RAWA DARI INDRALAYA
Authors: Baehaki, Ace
Rinto
Issue Date: 2012
Publisher: Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
Series/Report no.: Volume 15 Nomor 1;
Abstract: Proteases constitute one of the most important groups of industrial enzymes, therefore screening of these proteases from bacteria is important. The aim of this study was to characterize proteases from plant swamp bacteria. The result of proteolytic assays revealed three isolates possesed proteolytic index >1.00 (T1P4, H1P4 and K1P4). The optimum fermentation time of each isolate was 48 hours. The optimum pH of proteases from T1P4, H1P4 and K1P4 was 8.0; 7.5; and 8.0, respectively. The optimum temperature of T1P4, H1P4 and K1P4 protease was 50 °C; 40 °C; and 50 °C, respectively. Metal ions (Fe, K, Mn, and Zn) inhibited T1P4 protease except Fe2+ (5 mM), H1P4 protease except Zn2+ (5 mM), K1P4 protease except Fe2+ (1 and 5 mM). Effect of inhibtor specific (EDTA) shown EDTA inhibit all proteases. Study on the effect of metals ion and spesific inhibitors indicated that all protease were metaloprotease. The moleculer weights of T1P4, H1P4 and K1P4 proteases were determinited by using SDS-PAGE and zymography technique were 151; 138; and 127 kD, respectively.
URI: http://repository.ipb.ac.id/handle/123456789/77097
ISSN: 0854-9230
Appears in Collections:Jurnal Pengolahan Hasil Perikanan Indonesia

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