IDENTIFIKASI KERAGAMAN GEN -KASEIN (CSN2) PADA KAMBING PERANAKAN ETAWAH, SAANEN DAN PERSILANGANNYA DENGAN METODE PCR-SSCP

Abstract

β-casein gene is directly related to the quality and properties of milk. A protocol for rapid and simultaneous genotyping of β-kasein alleles in goat was conducted by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) method. Screening of β-kasein gene variability in 3 dairy goat breeds was conducted to: Etawah Grade (77 samples), Saanen (67 samples) and PESA (Crossbreed Etawah Grade with Saanen) (29 samples) in Bogor and Sukabumi. The objective of this research was to identify polymorphism of the β-kasein (CSN2) gene in dairy goat. Result showed that three alleles of the β- casein gene is CSN2*A, CSN2*C, dan CSN2*O. In most breeds, CSN2*O occurred in the lowest frequency. The identification of the CSN2 gene variability in the goat breeds indicated the predominance of the A allele. The CSN2*A allele had a high frequency in Saanen in Cijeruk (0.66); Etawah Grade in Cariu (0.62); and PESA in Cariu (0.54). While the CSN2*C allele had a high frequency in PESA in Balitnak (0.83); Etawah Grade in Ciapus (0.48); and Saanen in Taurus (0.38). Based on the result of chi-square analysis, it was found out that Saanen in Cariu and Taurus was not in Hardy-Weinberg equilibrium.