PROCESS DESIGN OF PEPTONEPRODUCTION FROM PEANUT MEAL AS BYPRODUCT OF PEANUT OIL INDUSTRY USING CRUDE PAPAIN

Abstract

Peptone is an important component in the microbial growth media which has function as the source of amino acids. Up until now, Indonesia still importS peptone which reaches $17.84 milion per year in the last five years. That fact is the reason to develnp the research in peptone production by using protein source material thae is available in Indonesia, such as peanut meal that can be obtained as by produce of peanut oil industry. 'Ihe objectives of ehis research was to design the production process of peanm peptone using enzymatic hydrolysis by crude papain. Design process was performed by determination of the best condition (time of hydrolysis, crude papain concentration and temperature of hydrolysis. Peanut pep cone produced was characterized on its amino acids content and applied co be used as microbial grO\vch media and compared with a commercial product. Crude papain powder was obtained from the sap of papaya fruit, through drying and milling proct:ss. TIle acrivicy of ccude papain used in this experiment was 5057.47 U/g min. Hydrolysis process was conducted on peanut meal that have been diluted in water with racio of 1 :2. TIll! rt:search showed that tht: best hydrolysis condition of peanut peprone was obtained by using 0.4% of crude papain for 4 h at temper~lture of 55 "C. "[he peptone product has appearance of brownish yellow color in liquid form. lhe yield pepmne produced was 40.8%. The analysis of amino acids coment showed that peanut peprone produced had high glutamic acid aspartic acid and arginine. TIlOse 3 amino acids are very impof(an[ for microbial growth. The result of growth test of Escherichia coli and Staphylococcus aurcus by determining optical densicy (OD620nm) and Toral Plate Count showed that peanuc peptone produced has similar performance with commercial peptone.