Cyclo (Tyrosyl-Prolyl) Produced by sp.: Bioactivity and Molecular Structure Elucidation Streptomyces

Abstract

Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by sp. have been carried out. The antibiotic was produced by liquid culture using sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, H NMR, C NMR, and C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to ATCC 25922, ATCC27853 , ATCC25923, and ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg mL , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol , and molecular formula was C H N O . Elucidation of molecular structure using HNMR, C NMR, and C DEPTNMRshowed that antibiotic was cyclo(tyrosyl-prolyl).