Variasi Dahan Bioaktif dan Bioaktivitas Tiga Nomor Harapan Temulawak pada Lokasi Budidaya Berbeda

Abstract

Temulawak (Curcuma xanthorrhiza Roxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1, 1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0. 056 g plant1 of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1 and LC50 of toxicity was 69.05 mg L-'. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 °C, rainfall± 223.97 mm year1 and sandy clay soil.