Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/72864
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Falah, Syamsul | |
dc.contributor.advisor | Khusniati, Tatik | |
dc.contributor.author | Fury, Bellen Nastitie Pamela | |
dc.date.accessioned | 2015-01-05T01:51:04Z | |
dc.date.available | 2015-01-05T01:51:04Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/72864 | |
dc.description.abstract | Lactose intolerance was a problem faced by a large proportion of the world's population. These problem could be alleviated by hydrolyzing lactose to glucose and galactose by β-galactosidase. β-Galactosidase occurred widely in nature and it was produced by a number of microorganisms. However, their characteristics were very different and it varied with the source of microorganism. The aim of this study was purification and characterization of β-galactosidase L. plantarum B 123. β-Galactosidase was extracted and partially purified by ammonium sulfate precipitation and dialysis. These steps resulted a purification level of 7.61 fold, a yield of 10.67%, and a specific activity of 61.53 U/mg protein. Optimum temperature and pH for dialyzed β-galactosidase were optimized at 50 °C and 7.0, respectively. Metal ions of Mg2+, Mn2+, Co2+, and Zn2+ in 10 mM stimulated the activity of crude β-galactosidase, while metal ions of Cu2+, Hg2+, and Ca2+ in 10 mM inhibited the activity of crude β-galactosidase. KM and Vmax for this dialyzed β-galactosidase were 1.13 mM and 7.25 U/mL, respectively. | en |
dc.language.iso | id | |
dc.subject.ddc | Lactobacillus plantarum B 123 | en |
dc.subject.ddc | Biochemistry | en |
dc.title | Pemurnian Parsial dan Karakterisasi Beta-Galaktosidase Lactobacillus plantarum B 123 | en |
dc.subject.keyword | Bogor Agricultural University (IPB) | en |
dc.subject.keyword | characterization | en |
dc.subject.keyword | dialysis | en |
dc.subject.keyword | Lactobacillus plantarum B 123 | en |
dc.subject.keyword | β-galactosidase | en |
Appears in Collections: | UT - Biochemistry |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
G14bnp.pdf Restricted Access | full text | 9.42 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.