Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/72103
Title: Produksi rekombinan plantaricin yang mengkode Bakteriosin dari lactobacillus plantarum s34 asal isolat Bekasem daging sapi untuk menanggulangi Demam typhoid
Other Titles: Prosiding Seminar Hasil-Hasil Penelitian IPB 2012
Authors: Suryani
Mustopa, A. Zaenal
Sukmarini, Linda
Adawiyah, Rabiatul
Hasim
Issue Date: 2012
Abstract: Peptida antimikroba dari bakteriosin yang dihasilkan dari bakteri asam laktat potensial untuk diterapkan pada industri pangan dan farmasi. Karakteristik bakteriosin kelas I dan II yang tahan panas sangat potensial untuk diaplikasikan pada industri. Bakteriosin dari L. plantarum S34 (plantaricin S34) yang di isolasi dari bekasam, fermentasi daging dari Lampung mempunyai potensi dalam menghambat bakteri patogen seperti Salmonella typhy dan Listeria monocytogenes. Bakteriosin tersebut stabil terhadap panas, berukuran 2,89 dan 8,99 kDa. Tujuan penelitian ini adalah melakukan isolasi dan karakterisasi gen plantaricin dari L. plantarum S34. Gen plantaricin diamplifikasi dari DNA genom L. plantarum S34 dengan primer spesifik menggunakan teknik Polymerase Chain Reaction (PCR). Hasil nya menunjukan 3 gen plantaricin EF, JK dan NC8 berhasil di isolasi dan dikarakterisasi. Analisis BLAST menunjukan gen plantaricin EF, JK, dan NC8 dari L.plantarum S34 masing-masing tersusun atas 285 bp, 267 bp dan 200 bp. Sedangkan analisis susunan asam amino plantaricin EF, JK dan NC8 masing-masing sebanyak 52, 57 dan 47 asam amino. Gen plantaricin EF sudah berhasil di kloning ke pGEMTeasy vector selanjutnya akan di subklon ke pET system untuk melihat ekspresi dari plantaricin tersebut. Untuk mengembangkan obat antimikroba yang aman bagi industri farmasi, maka pada penelitian ini akan dilakukan produksi rekombinan plantaricin pada bakteri E.coli.
Antimicrobial peptides of bacteriocins from lactic acid bacteria have received particular attention due to their potential application in the food industry and pharmaceutical. Among bacteriocins produced by lactic acid bacteria, the Class I and II have the best potential for industrial application with their small-heat stable cationic peptides. Bacteriocin from L.plantarum S34 (plantaricin S34) with high antimicrobial activity to pathogenic Salmonella typhy and Listeria monocytogenes has been isolated from bekasem, a traditional fermented meat from Lampung, Indonesia. The molecular weight of 2,89 kDa and 8,9 kDa heat stable-peptide plantaricin S34 has been identified as a Class I and II bacteriocin. In the present study, the isolation and characterization of plantaricin gene from L.plantarum S34 has been conducted. The plantaricin gene has been amplified from genom L.plantarum S34 with specific primer using Polymerase Chain Reaction (PCR). The results of BLAST analysis showed that plantaricin EF, JK and NC8 genes isolated from the L.plantarum S34 were 285 bp, 267 bp and 200 bp respectively. The amino acid also showed that plantaricin encoded by the plantaricin EF, JK and NC8 genes consisted of 52 amino acids, 57 amino acids and 47 amino acids, respectively. A 365 bp of PCR product plnEF has been cloned into pGEM-T Easy vector and transformed into Escherichia coli DH5α. Further, the gene fragment encoding mature plantaricin EF will be expressed in Escherichia coli BL21 using pET vector system.
URI: http://repository.ipb.ac.id/handle/123456789/72103
Appears in Collections:Research Proceeding

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