Penapisan Bakteri Rhizosfer Padi Penghasil Nitrogenase yang Tahan Kondisi Salin dan Identifikasi Gen nifH

Abstract

The ability of nitrogen fixing by rhizosfer bacteria could be used to decrease salinity impact in coastal rice field. It was caused by nitrogenase, encoded by nifH gene in component II. The objectives of this research were to screen of rhizosfer bacterias that have the nitrogenase activity in saline condition and identify the presence of nifH gene. The fifty isolates of rhizosfer bacteri isolated from coastal rice field Eretan and Patimban region, West Java was analyzed. Five isolates showed the nitrogenase activity under saline condition, are Er B1 3, Er B1 4, Er B1 9, Er B2 10 dan Ptb B1 4. The identification of nifH gene from these 5 samples using polymerase chain reaction (PCR) showed that the size is ~360 bp. The highest activity of nitrogenase used acetylene reduction assay (ARA) was showed by Er B2 10 is 4,4081 μmol.mL-1hour-1.