Effect of Catfish (Clarias gariepinus) Flour, Oil and Probiotic Based Feed Provision on Bodyweight, Lipid Profile and C-Reactive Protein of Aged Female Cynomolgus Monkey.

Abstract

Nowadays population of elderly people are increasing. The elderly are one among vulnerable groups due to weakening of immune. The aging process is related to the changes of BMI (Body Mass Index). The increase of obesity on the elderly is followed by the increase of prevalence of chronic diseases such as atherosclerocis, cognitive impairement, diabetic, hypertention, heart disease. Hence, alternative formulated specific food for elderly is urgently needed. The aim of research was to study the effect of catfish’ (Clarias gariepinus) flour, oil and probiotic E. faecium IS-27526 based feed provision on bodyweight, lipid profile and C-Reactive Protein (CRP) of aged female cynomolgus monkey (Macaca fascicularis). This study was conducted from March to august 2013 in Primate Research Center Bogor Agricultural University, Microbiology laboratory of Seafast and Atmajaya University, PT Carmelitha Lestari. This study consisted of two phases: a preliminary phase and the main phase. Preparation of animal and diet was conducted in preliminary phase. The age was determined by dentition, with body weight in a range of 2 – 4 kg. Animals were placed in the individual cages in the position where they can interact audiovisually. Nine aged female cynolmolgus monkeys were randomly divided into three groups, namely: standard diet (A1) group, standard diet + probiotic Enterococcus faecium IS-27526 (A2) group, standard diet + probiotic Enterococcus faecium IS-27526 + catfish oil (A3) group. Evaluation of bodyweight, blood lipid profile, and CRP were conducted monthly during 3 months intervention study. During intervention, there was no significant effect (p>0.05) of experimental diets on body weight in each group. However, probiotic tended to reduce the overweight risk. Total cholesterol and LDL cholesterol levels of monkeys with probiotic diet tended to be lower than other groups. There was no significant effect of the functional feed to triglyceride level and HDL cholesterol level, but the result showed monkeys fed with fish’ oil and probiotic E. faecium IS-27526 tended to decrease the triglyceride level compared with other groups. There was no effect of experimental diets on CRP which is marked by negative result of CRP test. Taken together, provision of probiotic diet (A2) reduced increment of body weight. Catfish oil tended to delay increment of trigliceryde. This study was expected to have a positive impact when applied to the elderly.