Ekstraksi dan Karakterisasi Kolagen dari Kulit Ikan Cobia (Rachycentron canadum)

Abstract

Cobia fish (Ranchycentron canadum) was used for a culinary purpose, but their skin was waste part. Convert in a value added product like collagen was a good practice of post harvest management. Isolation of collagen from the skin of cobia fish (Rachycentron canadum) has been successfully conducted with three stages: first soaked in 0,05 M NaOH with a sample/solution ratio of 1:10 (w/v) for 6 hour, second soaked in 0,1 M acetic acid with a sample/solution ratio of 1:6 (w/v) for 2 hour, and third extraction with destilated water at temperature 40 ºC for 3 hour. The yield of collagen was 10,508%. The pH of collagen was 7,08; protein content 86,46%; moisture 11,08%; fat 0,26%; and ash contents 0,19%. Amino acid analysis of collagen revealed the high proline and glycine. FTIR spectra analysis revealed that collagen molecules had the compact triple helical structure stabilized mainly by the hydrogen bond. Fourier transform infrared (FTIR) spectroscopic study showed major absorption bands of amide A, B, I, II and III at 3310, 2932, 1651, 1543 and 1242 cm-1 respectively.