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Title: | Kultur Biji Amorphophallus decus-silvae pada Beberapa Kombinasi Sitokinin dan Auksin secara in vitro |
Authors: | Dinarti, Diny Wirianto, Indra |
Issue Date: | 2014 |
Abstract: | Amorphophallus decus-silvae is focused in this research study specifically in plant growth regulator for seed germination and shoot production. The statistic model used was completely randomized design one factor with 9 medium composition with 0.5 MS basal medium + B5. Each medium had 20 bottle culture and totally 180 explants culture. The explants used were 3-4 week after pollination. In Amorphophallus decus-silvae seed tissue culture, 81.67 % sterilization success by using a method of seed immersion for 1 minute in antiseptic liquid and 15 minute in bleach 15 %. Medium 2 ppm BAP + 0.1 ppm IBA resulted in 94.1% explants germination. After 18 weeks planted in medium 2 ppm BAP + 0.1ppm IBA, the seed culture of Amophophallus decus-silvae produced 10.3 (top explants) and 7.3 (bottom explants) shoot. After 28 week, the total number of micro shoot are 30.4 in medium 2 ppm BAP + 0.1 ppm. Explants from 0.5 MS0 without growth regulator had 5.4 cm height after 28 week in 3 ppm GA3 medium. In acclimatization all plant (20 plantlet) could survived for 2 week observation |
URI: | http://repository.ipb.ac.id/handle/123456789/68093 |
Appears in Collections: | UT - Agronomy and Horticulture |
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File | Description | Size | Format | |
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A14iwi.pdf Restricted Access | full text | 980.75 kB | Adobe PDF | View/Open |
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