Identifikasi Senyawa Bioaktif Antifeedant dari Asap Cair Hasil Pirolisis Sampah Organik Perkotaan

Abstract

The research aims to identify antifeedant bioactive substance in liquid smoke derived from pyrolisis oforganic municipal waste. Samples were pyrolyzed in drum reactor at 505"C for five hours to produce char and conden~ed smoke turning into liquid smoke. The liquid smoke components are separated into n-heksan, etil asetat, metano! and water ccnsecutively. The result is thickened with rotary evaporator. The resulting crude's activity towards Spodoptera litura Linn larvae was tested with antifeedant method. The chemical components of active fraction are identified with GeMS. Crude resuiting from thickening fractions ofn-heksan, eti! asetat, metanol and water are 0.3465 g, 2.3736 g; 0.8775 g and 0.3679 g consecutively. The highest activity ofliquid smoke, fractions of"vater, methanol, etil asetat and J1-hek:;an towards S. /itllra larvae at 1.00% concentration are 44.68%; 62.07%; 80.65%; 28.57% and 23.40%. Result of probit analysis towards these fractions shuws that the highest actIvity is methanol fraction with EI50 value at 0.71. Identification with GCl'v1S shows that the fra('tions contain 14 types of bioactive antifeedant substances with y-butyrolacton as the main component.