Studi Komponen Aktif Temu Lawak terhadap Patogenesis Kanker Kolorektum Jalur Protein Induser dengan Penambatan Molekular

Abstract

Colorectal cancer is caused by excessive amount of inducible NOS (iNOS) and cyclooxygenase-2 (COX-2) protein in the body. Temu lawak is a plant potential as anticancer. The molecular docking method can be used to determine the activity and efficiency of the temu lawak bioactive compounds, which can inhibit the colorectal cancer through inducer protein iNOS and COX-2 pathway. Based on the molecular docking results of 24 bioactive compounds of temu lawak against 16 proteins in iNOS and COX-2 pathways, the two most potencial anticancer compounds were obtained, demethoxycurcumin and dihydrocurcumin. Both compounds could inhibit colorectal cancer through γ-interferon protein in iNOS pathway and inhibit mitogen activated protein kinase in COX-2 pathway. The experimental parameters used were Gibbs free energy (ΔG), affinity (pKi), inhibition constant (Ki), efficiency, and hydrogen bonding. Based on those parameters, the demethoxycurcumin and dihydrocurcumin were more potencial than simvastatin and rosmaniric acid standards.