Ekspresi Gen Sintetik Penyandi Parasporin-2 yang Diklon pada Plasmid pJExpress dalam Sel Inang Escherichia coli Top 10 F.

Abstract

Parasporin protein was produced by strains of Bacillus thuringiensis. Parasporin-2 is one of some types of parasporin protein which has the highest ability to kill cancer cells compared than the other types of parasporin. However, the natural strains of producing parasporin-2 requires a lot of time and also the operational cost was quite expensive. So, the synthetic gene encoder parasporin-2 should be created and cloned on pJExpress plasmid in Escherichia coli 10 F. The purpose of this research was to determine the best value of Escherichia coli 10 F optical density in order to get the optimal expression of recombinant protein of parasporin-2. Expression of protein recombinant was comparing the optimization of protein ekspression between IPTG 100 mM and 200 mM in induction and also the measurement of OD600 value in 0.4 and 0.6. The best result of the expression parasporin-2 sintetic gene was from OD600 0.4 with IPTG 100 mM.