Komposisi zat pengatur tumbuh untuk organogenesis dan induksi Kalus Pometia coriaceae secara In Vitro

Abstract

Development of Pometia coriaceae, wich is widely used by local community as timber crops of high economic value is still constrained by its propagation method. The difficulty in its natural reproduction can be overcome by tissue culture techniques. The success of tissue culture propagation depends on suitable sterilization techniques, growth media, as well as the composition of growth regulators used. Thus it is important to find suitable sterilization techniques, growth media and plant growth regulators for in vitro propagation system of P. coriaceae. The purpose of this study were to obtain suitable sterilization techniques, which consisted of combination of bactericide and fungicide concentration and immersion time for P. coriaceae explants, to find suitable media composition containing composition of growth regulators concentration for explants multiplication of through organogenesis and embryogenic callus induction. A total of 4 kinds of sterilization treatment, which consisted of combination of 2 different concentrations of bactericide and fungicide with 2 different immersion times were tested on embryo, shoot of seedling, leaf and endosperm explants. For induction of organogenesis 4 kinds of composition of MS medium containing different concentrations of NAA and kinetin were tested on embryo and shoot explants. Callus induction was done on MS medium containing 4 compositions of NAA and kinetin for leaf and endosperm explants. The results showed that the best sterilization technique for sterilizing P. coriaceae explants was by immersing explants in a solution composed of bactericid and fungicide each 0.4 g/100mL for 5 minutes. MS medium fortified with 1.5 ppm NAA and 2 ppm kinetin was the medium for organogenesis of P. coriaceae embryo explants. MS0 medium, MS medium containing 0.5 ppm NAA and 1 ppm kinetin, or combination of 1 ppm NAA and 1.5 ppm kinetin, or combination of 1.5 ppm NAA and 2 ppm kinetin were able to induce callus formation from endosperm explants with the formation of roots. Callus formed on MS medium enriched with 2 ppm NAA and 2.5 ppm kinetin from endosperm has potential as embryogenic callus.