Alkaloid Extract from Crown of God Fruit as α-Glucosidase Inhibitor

Abstract

The crown of God Fruit is believed to cure diabetes mellitus. The objective of this study is to investigate the highest inhibitory activity of α-glucosidase from alkaloid extract of the fruit by in vitro method. Three levels of ripeness used in this study i.e. dark red, red-greenish, and green-reddish. The result showed that alkaloid from red-greenish crown of god fruit extract had the highest inhibition activity of 36.80%, but was lower than acarbose which has 97.73%. Fractionation of alkaloid extract obtained 5 fractions from red-greenish crown of god fruit was done by column cromatography with the eluent of chloroform:ethanol (17:3). Inhibiton activity of each fractions to α-glucosidase was examined and it was obtained that the most active fraction was fraction 5 with inhibition activity value of 87.29%. Further identification of fraction 5 with infrared spectrophotometrer showed the presence of possible functional groups; there were –OH stretching at 3408.87 cm-1 of wave-number, C=C aromatic stretching at 1616.91 cm-1 and C-N amine aromatic stetching at 1279.88 cm-1, which was strengthened by overtone band at 617.21 cm-1. Based on those result, it could be predicted that the alkaloid compound was alkaloid with nitrogen atom in the aromatic ring.