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Title: | N2 fixation and methane oxidation effectiveness of mixed cultures of methanotrophic bacteria from ricefields Efektivitas fiksasi N2 dan oksidasi metan kultur campuran bakteri metanotrof asal sawah |
Authors: | Meryandini, Anja Sunarti, Titi Candra Chatrinan, Vina |
Keywords: | Methane methanotrophic bacteria ARA nitrogen fixation methane oxidation |
Issue Date: | 2010 |
Abstract: | CH4 emissions in a rice field and wetlands is mostly produced by methanogenic bacteria and 80% of the CH4 is oxidized by methanotrophs. Besides of their ability to oxidize methane, methanotrophs also have ability to fix nitrogen. Four selected isolates of methanotrophs which had high nitrogen fixation activity i.e.(BGM 1, BGM 3, BGM 5, and BGM 9 isolates were combined with SKM 14 isolate which had high methane oxidation activity to obtain ideal formulations of the methanotrophs that have high activity of nitrogen fixation and methane oxidation activities. Nitrogen fixation activity was determined by measuring accumulated ammonium in medium and acetylene reduction assay (ARA). The highest concentraion of ammonium on single culture of methanotrophic bacterium was performed BGM 1 isolate (1,86 μM ammonium), and mixed culture of BGM3 and BGM9 isolates had the highest ammonium accumulation (4,905 μM ammonium). The highest nitrogenase activity was performed by mixed culture of BGM1 and SKM14 isolates, it was 11,27 μmol/ml cultures/hour. Whereas the higest methane oxidation was performed by mixed culture of BGM5 and BGM9 isolates, it was 18,20 ppm /ml cultures /day. |
URI: | http://repository.ipb.ac.id/handle/123456789/62158 |
Appears in Collections: | UT - Biology |
Files in This Item:
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G10vch.pdf Restricted Access | Fulltext | 738.97 kB | Adobe PDF | View/Open |
Abstract.pdf Restricted Access | Abstract | 283.63 kB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 285.71 kB | Adobe PDF | View/Open |
BAB II Bahan dan Metode.pdf Restricted Access | BAB III | 556.2 kB | Adobe PDF | View/Open |
BAB III Hasil dan Pembahasan.pdf Restricted Access | BAB III | 512.41 kB | Adobe PDF | View/Open |
BAB IV Simpulan dan Saran.pdf Restricted Access | BAB IV | 292.43 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 288.55 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 284.36 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 404.5 kB | Adobe PDF | View/Open |
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