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Title: | Haploidisasi melalui androgenesis dan ginogenesis pada anyelir (Dianthus sp.) Haploidization through androgenesis and gynogenesis on carnation (Dianthus sp.). |
Authors: | Purwito, Agus G.A. Wattimena Marwoto, Budi Sukma, Dewi Kartikaningrum, Suskandari |
Keywords: | androgenesis gynogenesis pseudofertilization haploid Dianthus sp |
Issue Date: | 2013 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Haploidization technology ensures important advantages in obtaining pure lines by rapid fixation of homozygosity. Anther culture, ovule culture, ovary culture and irradiated pollen technique were used in this study. The objective of the research was to develop appropriate haploid technology in order to obtain haploid plants through androgenesis, gynogenesis by ovule culture and ovary culture and pseudofertilization on Dianthus chienesis. Androgenic callus was induced in four WT basic medium inductions supplemented with 2.4D, NAA, TDZ and BA. Four explants originated from ovule and ovary cultures of six genotypes of D. chinensis (Dchi-11, Dchi-12, Dchi-13, Dchi-14, Dchi-15 and Dchi-16) were applied in five media. For the parthenogenesis induction, 100-200 Gray gamma irradiations were applied to the Dchi-14 pollens and pollinated to the Dchi-11. Pollinations were conducted on the day after irradiation. Eight media were selected to obtain regenerated plants. Results showed that androgenic callus formation needed high auxin and sitokinin ratio, while regenerated callus required lower auxin and sitokinin ratio. 2,4-D was better than NAA in callus induction. Callus originated from anther culture, multi ovule slice culture, ovule culture and ovary slice culture regenerated producing early flowering plants and expressed of abnormal flowering mutant. Regeneran from anther culture was diploid, but two putative double haploids came up from multi ovule slice culture and ovary culture. Cytology and flow cytometry observations showed that seven haploid plants were obtained from pseudofertilization. Spontaneous chromosome doubling was inferred to have occurred during the callus culture period. In conclusion, gynogenesis through multiovule culture, ovary slice culture and pseudofertilization was more effective in inducing haploid and double haploid plants than androgenesis. |
URI: | http://repository.ipb.ac.id/handle/123456789/61986 |
Appears in Collections: | DT - Agriculture |
Files in This Item:
File | Description | Size | Format | |
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2013ska.pdf Restricted Access | Fulltext | 2.01 MB | Adobe PDF | View/Open |
Abstract.pdf Restricted Access | Abstract | 372.13 kB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 472.51 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 422.06 kB | Adobe PDF | View/Open |
BAB III Studi Tahap Perkembangan Kuncup Bunga ....pdf Restricted Access | BAB III | 658.28 kB | Adobe PDF | View/Open |
BAB IV Induksi Haploid Dianthus Chinensis ....pdf Restricted Access | BAB IV | 547.79 kB | Adobe PDF | View/Open |
BAB V Induksi Haploid Dianthus Chinensis ....pdf Restricted Access | BAB V | 783.71 kB | Adobe PDF | View/Open |
BAB VI Induksi Tanaman Haploid ....pdf Restricted Access | BAB VI | 852.65 kB | Adobe PDF | View/Open |
BAB VII Pembahasan Umum.pdf Restricted Access | BAB VII | 421.26 kB | Adobe PDF | View/Open |
BAB VIII Simpulan dan Saran.pdf Restricted Access | BAB VIII | 377.69 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 311.78 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 441.23 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 396.62 kB | Adobe PDF | View/Open |
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