In vitro determination of antioxidant activity of extracts of mahkota dewa, temu putih, sambiloto and keladi tikus

Abstract

'vfahkota dewa (Phaleria macrocarpa, Boer!), lemu pUlih (Curcuma zeodaria), sambilolo (Andrographl:~ panteu/ala, Nees) , and Ice/adi lilcus (Typhonium/lagelliji)rme) are medicinal plants that have potency as antioxidant and anticancer. The objective of this study was to determine the antioxidant capability of extract of mahkola dewa. temu pulih, sambilolo, and ke/adi tikus using tiobarbituric acid method. In this method, linoleic acid was oxidized by oxygen on 40" C for 8 days and produced malondialdehyde. The corresponding malondialdehyde have reacted with tiobarbituric acid and produced red product, the absorbance was measured on wavelength of 532 nm. The antioxidant potency of all plant extracts was monitored through its capability on inhibiting oxidation. Inhibition capability of the respective extracts on concentration of 200 ppm for aquademineralized, hot water, and ethanol extract were 83.44, 70.86, and 81.84% (mahlcota dewa), 60.21, 82.74, and 69.28% (Iemu pUlih), 81.45, 81.45, and 67.96% (sambi/olo). 68.60, 63.53, and 72.17% (ke/adi tikus), plus 87.0 I % (vitamin E), respectively. Based on analysis of variance and Duncan's test, antioxidant potency of all of extracts at concentration of200 ppm, if compared with vitamin E, were significantly different on confidence level of95%.