Pembentukan Blastosis Pada Embrio Sapi yang Difertilisasi Secara In Vitro dengan Semen Sapi Bali (Bos javanicus) dan Semen Sapi Simmental (Bos taurus)

Abstract

In vitro fertilization is one of reproductive biotechnology which can be used to increase population, productivity, and quality of cattle in Indonesia. Bali cattle (Bos javanicus) is one of native cattle from Indonesia, which have good carcass, meat production, which has adapted with invironment, and also have good fertility. Development of Bali cattle population can be accived through Embryo Transfer (ET) using IVF embryo production. The aim of this study was to determine the ability of Bali cattle semen (Bos javanicus) and compared with Simmental cattle semen (Bos taurus) to fertilized and its effect on cleavage rate until the blastocyst stage. This study was conducted in May and June 2012 at laboratory IVF production of Balai Embrio Ternak Cipelang, Bogor. Matured oocytes will be fertilized using Bali cattle semen and Simmental cattle semen, which have been capacitated before. Comparisons between bulls for cleavage, morula, and blastocyst rate, were analyzed using Chi-square analysis. The results indicate that Bali cattle can produced higher cleavage rate, morula rate, and blastocyst rate compared with Simmental cattle for in vitro embryo production. Embryo cleavage rate, morula rate and blastocyst rate were significantly different (P>0,05). The competence of embryo development from the blastocyst rate at day 6 was significantly different (P<0,05) but blastocyst rate at day 7, 8, and 9 were not significantly different (P>0,05).