Studi Histopatologi Ginjal Tikus yang Diinduksi Etilen Glikol: Pengaruh Infusum Daun Alpukat (Persea americana Mill.).

Abstract

The objective of this research was to study the histopathological lesions of rat kidneys induced by ethylene glycol and treated by avocado leaves infusum. This research is continuation of Adha’s experiment (2009) with the experimental design as follows: twenty male rats were divided into four groups, namely the control negative (KN) were received just drinking water ad libitum, the positive control (KP) were induced by 0.75% ethylen glycol and 2% ammonium chloride (inducer), the treatment group I (IF5) were induced and treated by 5% avocado leaves infusum at a dose of 3 ml/200 g BW,and the treatment group II (IF 10) were induced and treated by 10% avocado leaves infusum (3 ml/200 g BW). Treatment was given for 10 days, at day-11 rats were euthanasid, and kidney organs were taken for histopathological study and stained with Hematoxylin- Eosin. The histopatological study showed glomerular edema; hyaline droplet, deposition of protein and tubular necrosis. Percentage of glomerular edema of all treatment groups were not significantly different (p>0.05) compared with negative control group, while the percentage of tubular necrosis of positive control group was significantly different (p<0.05) compared with negative control group. Treatment with avocado leaves infusum can reduce the percentage of necrotic tubulus whereas doses of infusum tend to decrease precentage in protein deposition, hyaline droplet and tubular necrosis. Percentage decrease in tubular damage caused by the avocado leaves flavonoid activity as diureticum and antioxidants.