Study of Biphasic Calcium Phosphate Ceramics and HA-Chitosan Composite Implanted into Sheep’s Bone

Abstract

Biphasic Calcium Phosphate (BCP) biomaterials are recognized as biodegradable, bioresorbable, and osteoconductive material and, therefore, it is suitable for filling bone defects to aid regeneration process of new bone tissue. This study aimed to synthesize BCP ceramics by precipitation method and observe bone growth using BCP ceramics and HA-Chitosan composite implanted into sheep’s bone. BCP ceramics was prepared by precipitation of Na2HPO4.2H2O which was dropped by CaCl2.2H2O at temperature 700C with various molar ratios and various volume of solution. Molar ratio of Ca and P was variated approximately 0.2M:0.2M and 0.334M:0.2M while various volume of solution was 50 ml and 100 ml. BCP ceramics was formed which was indicated by mass of precipitate and the result showed that sample with greater molarity of Ca was resulting in bigger mass precipitate. It showed that higher molarity of Ca/P tends to be more mass precipitate. The X-Ray Diffraction results showed that BCP 100 ml with molar ratio Ca/P about 0.334M:0.2M had the expected characteristics of implant material for in vivo experiment into sheep’s bone. Moreover, it showed the consistency of sample which indicates that it was a reproducible biomaterial. This result was also supported by Fourier Transform Infrared (FTIR) and Scanning Electron Microscopy (SEM) characterization. In vivo study of BCP ceramics and HA-Chitosan composite showed that bone recovery without bone graft (control) was more complete than that with BCP and HA-Chitosan bone grafts. Based on pathological evaluation, the results indicated that BCP ceramics was biodegradable and bioresorbable but less osteoconductive. In the contrary, HA-chitosan composite was not biodegradable and bioresorbable but more osteoconductive. Moreover, BCP bone graft was more readily resorbed by the body than HAChitosan. Resorption of HA-Chitosan bone graft was very slow but new tissue growth was faster than that of BCP bone graft. In contrary, resorption of BCP bone graft was very fast but new tissue growth was very slow.