In vitro anticestode activity of painted nettle leaves extract to hymenolepis microstoma: observation

Abstract

Study in uitro on the anticestodal activity of Coleus blu111ei leaves extracts against Hy111e11olepis 111icrosfolll11 was conducted. Leaves of C. blwuci were collected and extracted ·with hexane, chloroform, ethanol and water. Anthelmintic activity was evaluated with an assay using H. 111icrosfolllll in a serial microplate dilution method by determination of mortality time. The parasites were treated with varying concentrations, 10, 5, 2.5, 1, and 0.5 % of the plant extract. Scanning electron microscope analysis was performed to determine effect of painted nettle extract on the tegument and other superficial body ch<mges of H. wicrosto11111. The result of this study showed extracts fraction of semipolar solvent ( ethanolic and clorofonn extracts displayed strong anthelmintic activity with the highest activity belong to ethanolic extract. In general, ethanolic extract proved to be a more efficient extractant of biologically active compounds than either cloroform, hexane or aquous extract. On the assessment of the motility and mortality of the worms, a concentration-dependent activity of the plant extract was dearly discenlible. In the scanning electron microscopy, extensive distortion and destruction on the surface fine topography of the tegument were evident. Thus, the present experiment provides the plausibility of C. /1/w11ci leaves as an anthelmintic agent. The promising activity displayed by ethanolic extracts has led to further investigation of the activ~ compound, toxicity of the extract and in view efficacy.