Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/59171
Title: | Kultur In Vitro Sel-sel Fibroblas Fetus Tikus |
Authors: | Djuwita,Ita Harlystiarin |
Keywords: | Bogor Agricultural University (IPB) SDS PAGE cell line, fibroblast cell culture |
Issue Date: | 2010 |
Abstract: | Research has been conducted on the in vitro culture of rat fetal fibroblast using Dulbeccos’ Modified Eagle Medium supplemented with 3mM sodium bicarbonate, 10% fetal calf serum and 50 μg/ml gentamycin (mDMEM) in CO2 incubator at 37oC. Evaluation was done on the proliferation rate and the protein production after several passages. The growth of the fibroblast was confirmed by their morphology. The proliferation rate was done based on the population doubling time (PDT) by counting the number of cells in each passage by using Improved Neubauer hemocytometer. Identification of proteins was done quatitatively using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Based on morphological observation the cell grown in in vitro culture were the fibrocyte and fibroblast and showed increasing of homogenicity after several passages. Based on the PDT, the proliferation rate was increased by each passages. Fibroblast also secrete proteins remain until passage 9 based on the results of electrophoresis. It can be concluded that the growth and protein secretion of rat fetal fibroblast in in vitro culture can be maintained up to 9 passages. |
URI: | http://repository.ipb.ac.id/handle/123456789/59171 |
Appears in Collections: | UT - Anatomy, Phisiology and Pharmacology |
Files in This Item:
File | Description | Size | Format | |
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Abstract.pdf Restricted Access | Abstract | 298.75 kB | Adobe PDF | View/Open |
B10har.pdf Restricted Access | full text | 4.19 MB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 301.48 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 1.45 MB | Adobe PDF | View/Open |
BAB III Metodologi.pdf Restricted Access | BAB III | 846.02 kB | Adobe PDF | View/Open |
BAB IV Hasil dan Pembahasan.pdf Restricted Access | BAB IV | 2.39 MB | Adobe PDF | View/Open |
BAB V Simpulan dan Saran.pdf Restricted Access | BAB V | 292.79 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 318 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 306.63 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 303.65 kB | Adobe PDF | View/Open |
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