Kajian penanda genetik gen cytochrome B pada Tarsius sp

Abstract

Tarsius is an endemic species in Indonesia that is threatened. Conservation of this species would yield better results if its genetic make up and diversity were determined. The objective of this research was to study the specific genetic marker on cyt b gene of Tarsius sp. Sequencing of PCR product using primer H15149 on cyt b gene yielded base sequence of 276 bp (coding 92 amino acids). Multiple alignment was carried out using Genetyc-Win Version 3.0 and Clustal W andlyzed using MEGA program version 3.1. Fourteen different amino acid sites were found. Tarsius dianae had 12 amino acid sites (amino acid no. 2, 6, 9, 22, 23. 29. 39, 41, 42, 45, 55 and 85), T. spectrum had 7 amino acid sites sites (amino acid no. 2, 6, 9, 41, 45, 55 and 85) and T. bancanus had 2 amino acid sites (amino acid no. 23 and 45) which can be used as genetic marker. Five uniqea amino acids were found on T. dianae, such as amino acid site: 6 (valine), 22 (alanin), 29 (alanin), 39(serin) and site 42(valin). The genetic distance based on Kimura 2-parameter model ranged between 0,7% and 22,3%, with average of 13.1%. The phylogram using neighbor Joining method on the sequence of nucleotide and amino acid of cyt b reveded differentiation among Tarsius.