Isolation Of Enterobacter Sakazakii (Cronobacter Spp) From Powdered Infant Formula And Other Dry Foods Obtained From Bogor Area, Indonesia

Abstract

Recently there has been increasing concern related to the presence of Enterobacter sakazakii in powdered infant formula (PIF) which was linked to severe systemic infection in certain groups of neonates. E.sakazakii has been previously isolated from PIF in Indonesia; however more information regarding E.sakazakii is needed to assess the risk of this pathogen for infants in Indonesia. The research was aimed to isolate E.sakazakii fromPIF, weaning foods and other dry foods obtained in Bogor, Indonesia. PIF and weaning food samples were obtained in 2006 and sampling were repeated in 2009. Several non PIF samples were collected in 2009. Isolation and detection were carried out according to FDA methods (2002) as modified by Iversen & Forsythe (2004). Four E. sakazakii were isolated from 2 out of 16 PIF samples in 2006 but none was isolated from 3 PIF samples collected in 2009. Four isolates were obtained from 2 out of 9 weaning foods samples obtained in 2006 while two were isolated from two out of 15 samples taken in 2009. Two E. sakazakii were also isolated from two corn starch samples and one was isolated from chocolate powder sample. The result implied significant improvement of E. sakazakii management by PIF manufacturer in the past 2 years, while improved processing and or process control is probably needed for dry food production to minimize contamination by E. sakazakii