Methanol extract derived from nias’s and mentawai’s sponges and soft corals as anti angiogenesis

Abstract

The exploration of anti angiogenesis potency from Mentawai and Nias's sponges and soft corals was studied. The anti angiogenesis test was conducted based on the population of endothelia cell line (CPAE, ATCC CCL). From 201 samples collected only two samples namely methanol extracts of soft coral GSO6 from Mentawai and sponge no. 18 from Nias. and their fractions were tested for anti angiogenesis. Fractionation for the two samples were done using silica gel column chromatography and gave 33 and 32 fractions respectively. The results indicated that methanol crude extract of sponge no. 18 was the active one. The fraction no. 16 extract 18 had 56 ppm of IC50 and IC50 of fraction no 6 GSO6 was 144 ppm. Further studied of active compounds for their pharmaceutical potency still are needed.