Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/58615
Title: | Potensi Pelepah Temulawak (Curcuma xanthorriza) sebagai Antikanker dan Antioksidan |
Authors: | Batubara, Irmanida Syahbirin, Gustini Septyanti, Cahya |
Keywords: | Bogor Agricultural University (IPB) |
Issue Date: | 2012 |
Abstract: | The study aimed to evaluate the potency of temulawak (Curcuma xanthorrhiza) stem as anticancer by brine shrimp lethality test (BSLT) and antioxidant by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The stem of temulawak midrib obtained from Bogor, was extracted by n-hexane, chloroform, ethyl acetate, methanol, and water, and the volatile oil was obtained by water distillation. The chloroform extract and volatile oil showed anticancer potency towards Artemia salina (LC50: 370.69 ± 22.69 μg mL-1 ; 123.05 ± 3.49 μg mL-1, respectively). Ethyl acetate and chloroform extracts had antioxidant activities (IC50: 46.41 ± 15.49 μg mL-1 and 52.72 ± 3.46 μg mL-1) but the activity did not as strong as ascorbic acid (control positive, IC50 1.80 ± 0.33 μg mL-1). The chloroform extract was further fractionated by column chromatography with Silica Gel G60F254-Merck as stationary phase and gradient of n-hexane, chloroform, and methanol as mobile phase. Antioxidant and anticancer potency of fraction which had antioxidant activity qualitatively were tested. The most antioxidant active fraction (F9) had anticancer potency (LC50: 522.95 ± 43.35 μg mL-1) and antioxidant activity (IC50: 79.57 ± 8.96 μg mL-1). In conclusion, F9 is potencial as an anticancer and antioxidant. |
URI: | http://repository.ipb.ac.id/handle/123456789/58615 |
Appears in Collections: | UT - Chemistry |
Files in This Item:
File | Size | Format | |
---|---|---|---|
G12cse.pdf Restricted Access | 1.43 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.