Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/58001
Title: | Penentuan Jenis Kelamin pada Kelas Aves Menggunakan Metode Polymerase Chain Reaction (PCR) |
Authors: | Jakaria Ulfah,Maria Khaerunnisa, Isyana |
Keywords: | Bogor Agricultural University (IPB) avian PCR CHD genes sex determination |
Issue Date: | 2012 |
Abstract: | Many avian species are sexually monomorphic. In this case, molecular approach is an efficient method for sex determination. The sexes of monomorphic avians can be determined by PCR amplification of the CHD genes. CHD genes are preserved within avian Z and W sex chromosomes. The objective of this research was to determine sex of chickens, quails, ducks, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos based on CHD gene using primer 2550F and 2718R. PCR products were screened by 1.5% agarose gel electrophoresis with Ethidium Bromide. According to the results 10 males and 11 females were determined from 21 specimens of avians. Individuals showed double (ZW) and single (ZZ) bands were identified as females and males, respectively in chickens, quails, hill myna, salmon-crested cockatoos and yellow-crested cockatoos. Males and females in ducks and pigeons showed single band in different length of basepairs. |
URI: | http://repository.ipb.ac.id/handle/123456789/58001 |
Appears in Collections: | UT - Animal Production Science and Technology |
Files in This Item:
File | Description | Size | Format | |
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Abstract.pdf Restricted Access | Abstract | 284.81 kB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 283.84 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 481.86 kB | Adobe PDF | View/Open |
BAB III Materi dan Metode.pdf Restricted Access | BAB III | 527.25 kB | Adobe PDF | View/Open |
BAB IV Hasil dan Pembahasan.pdf Restricted Access | BAB IV | 624.76 kB | Adobe PDF | View/Open |
BAB V Kesimpulan dan Saran.pdf Restricted Access | BAB V | 277.64 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 292.64 kB | Adobe PDF | View/Open |
D12ikh1.pdf Restricted Access | full text | 1.2 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 340.03 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 361.11 kB | Adobe PDF | View/Open |
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