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Title: | Isolasi Bakteri Proteolitik, Karakterisasi, dan Aplikasi Enzim Protease pada Kokon Cricula trifenestrata |
Authors: | Mubarik, Nisa Rachmania Solihin, Dedy Duryadi Gunawan, Abdi |
Keywords: | Bogor Agricultural University (IPB) goldsilk protease activity enzyme characterization isolation Cricula trifenestrata |
Issue Date: | 2012 |
Abstract: | Goldsilk is silk fiber that made by human which it get from Cricula trifenestrata cocoon. Existence of proteolitic bacterias from cocoon have potential to influence quality of goldsilk. The aims of the experiment were to get proteolytic bacteria, characterize the protease enzyme and apply protease on goldsilk cocoon. A total of 26 proteolytic bacteria were isolated from good and bad cocoons. Nutrient Broth (NB) and Nutrient Agar (NA) containing 1% skim milk used as growth media. Isolate CKH 7 and CKK 7 are selected for protease production because they have the highest protease activity. CKH 7 and CKK 7 were identified as Aeromonas salmonicida ssp salmonicida and Vibrio fluvialis. Highest protease productivity reached after 9 hour incubation. Optimum protease activity of isolate CKH 7 at pH 8 and temperature 25 °C. Its activity increased by the presence of 2 mM Na2+ and Ca2+. Activity isolate CKK 7 optimum showed at pH 5, reached after 12 hour incubation and temperature 35°C. Its activity increased by the presence of 2 mM Cu2+, Co2+ and Ca2+. Protease of CKH 7 characterized as metalloprotease. Result of submerge cocoon in protease and continued by NaOH 0,1 N are fiber be clean, shiny, and not easy cut off. |
URI: | http://repository.ipb.ac.id/handle/123456789/57739 |
Appears in Collections: | UT - Biology |
Files in This Item:
File | Description | Size | Format | |
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G12agu.pdf Restricted Access | Full text | 899.49 kB | Adobe PDF | View/Open |
G12agu_Abstrak.pdf Restricted Access | Abstrak | 285.65 kB | Adobe PDF | View/Open |
G12agu_BAB I Pendahuluan.pdf Restricted Access | BAB I | 290.4 kB | Adobe PDF | View/Open |
G12agu_BAB II Bahan dan Metode.pdf Restricted Access | BAB II | 290.11 kB | Adobe PDF | View/Open |
G12agu_BAB III Hasil.pdf Restricted Access | BAB III | 444.44 kB | Adobe PDF | View/Open |
G12agu_BAB IV Pembahasan.pdf Restricted Access | BAB IV | 329.48 kB | Adobe PDF | View/Open |
G12agu_Cover.pdf Restricted Access | Cover | 295.31 kB | Adobe PDF | View/Open |
G12agu_Lampiran.pdf Restricted Access | Lampiran | 696.31 kB | Adobe PDF | View/Open |
G12agu-Daftar Pustaka.pdf Restricted Access | daftar pustaka | 362.55 kB | Adobe PDF | View/Open |
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