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Title: | Purification and Characterization of Cellulase Enzyme from Bacteria Isolated from Seaweed Waste Pemurnian dan Karakterisasi Enzim Selulase dari Bakteri yang Diisolasi dari Limbah Rumput Laut |
Authors: | Mubarik, Nisa Rachmania Chasanah, Ekowati Rahmadini, Isna |
Keywords: | cellulase characterization purification seaweedwaste |
Issue Date: | 2012 |
Abstract: | Seaweedwasteis a sourceof bacteria thatcanproduce cellulaseenzyme. PMP0126yisolateis one collection isolateof the Research and Development Center for Marine and Fisheries Product Proecessing and Biotechnology Agency for Marine and Fisheries Research and Development Ministry of Marine Affairs and Fisheries obtainedfromseaweed wastefrom Pameungpeukarea, Garut, West Java. The aims this research were to purify, characterize the cellulase enzyme, and identify the bacteria producing the enzyme using 16S-rRNA. PMP 0126yisolatewasa Gram-negativeshortrodshape bacteria. Based on thesequencingof the 16S-rRNA genfrom 1282base pairPMP0126y isolate had96%similaritywith Chryseobacteriumindologenesstrain McR-1. Theisolate had 1.9 cellulolytic index onCarboxymethyl Cellulose(CMC) agar medium. The highestcellulaseactivityobtained onthe thirdday offermentation timewith acellulaseactivityof0.108U/mLandspecificactivityof0.120U/mg. Initialpurificationof cellulasebyultrafiltrationproducedactivityof0.112U/mL. Purificationthe enzyme byanionexchange chromatographyproducedthe highestpeak of proteininthefraction no. 48withcellulaseactivityof0.154U/mLat 37.3mMNaCl.Optimumactivity ofthe cellulaseenzymeafterultrafiltrationwaspH 5and300C,while optimumactivity ofthe cellulaseenzymebyanionexchange chromatographywaspH 5and400C. At 300C, the enzymeremainedstableup to4hourincubation.Theactivity ofthe cellulasewasincreasedbyaddition ofCaCl2ions by 53%anddecreased bythe additionof ZnCl2 ions by 78%. Thecellulase showed the highest activity i.e. 0.149U/mL using treated seaweed wasteGlacilariasp. as substrate. Using SDS-PAGE and zimogram analysis, the molecular weight of the cellulase was estimated to be 39 kDa, 30 kDa, and 14 kDa. |
URI: | http://repository.ipb.ac.id/handle/123456789/57518 |
Appears in Collections: | MT - Multidiciplinary Program |
Files in This Item:
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2012ira.pdf Restricted Access | Full text | 2.1 MB | Adobe PDF | View/Open |
2012ira_BAB I Pendahuluan.pdf Restricted Access | BAB I | 385.77 kB | Adobe PDF | View/Open |
2012ira_BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 625.31 kB | Adobe PDF | View/Open |
2012ira_BAB III Bahan dan Metode.pdf Restricted Access | BAB III | 440.34 kB | Adobe PDF | View/Open |
2012ira_BAB IV Hasil.pdf Restricted Access | BAB IV | 819.82 kB | Adobe PDF | View/Open |
2012ira_BAB V Pembahasan.pdf Restricted Access | BAB V | 426.83 kB | Adobe PDF | View/Open |
2012ira_BAB VI Simpulan.pdf Restricted Access | BAB VI | 333.55 kB | Adobe PDF | View/Open |
2012ira_Cover.pdf Restricted Access | Cover | 296.12 kB | Adobe PDF | View/Open |
2012ira_Daftar Pustaka.pdf Restricted Access | daftar pustaka | 452.02 kB | Adobe PDF | View/Open |
2012ira_Lampiran.pdf Restricted Access | lampiran | 1.09 MB | Adobe PDF | View/Open |
2012ira_Ringkasan.pdf Restricted Access | ringkasan | 282.06 kB | Adobe PDF | View/Open |
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