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Title: | Application of Recombinant Growth Hormone for Growth Stimulation in Giant Gourami Juvenile (Osphronemus goramy) by Immersion Aplikasi Hormon Pertumbuhan Rekombinan melalui Perendaman untuk Memacu Pertumbuhan Benih Ikan Gurami (Osphronemus goramy) |
Authors: | Alimuddin Sumantadinata, Komar Syazili, Aras |
Keywords: | Recombinant growth hormone immersion growth giant gourami |
Issue Date: | 2012 |
Abstract: | This study aimed to determine the immersion frequency of recombinant common carp (CcGH) and giant gourami (OgGH) growth hormone in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, were immersed in 30 ppt salt water for 2 minutes, then transferred into 200 mL of 9 ppt saline water containing 30 mg/L CcGH in inclusion bodies form. Immersion was performed for 60 minutes with a frequency of 2, 3, 4 and 5 times at interval 7 days. The results showed that the highest (p<0.05) average body weight of giant gourami juvenile (2.232±0.190 g) was obtained at 4 times immersion treatment. The total dosage of 4 times immersion (120 mg/L OgGH) from the first experiment was then used in the second experiment to determine whether immersion frequency could be minimized. Fish larvae were immersed once a week, twice a week, and once a week for 2 weeks. The results showed that the highest (p<0.05) biomass (5482.58±110.70 g), and survival rate (87.38±8.84%) were obtained by once immersion time. Hence, both CcGH and OgGH could improve growth of giant gourami juvenile. The IGF-I mRNA expression level was increased significantly at 24 hours after OgGH immersion, and this suggested that IGF-I was involved in growth stimulation. Lower plasma cortisol levels in OgGH-treated fish after 180 minutes may also plays important role in growth induction. Thus, immersion of juvenile once a week in 120 mg/L OgGH solution can be applied to enhance the growth of giant gourami with a high survival rate. |
URI: | http://repository.ipb.ac.id/handle/123456789/57470 |
Appears in Collections: | MT - Fisheries |
Files in This Item:
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2012asy.pdf Restricted Access | Full text | 972.21 kB | Adobe PDF | View/Open |
2012asy_Abstrak.pdf Restricted Access | Abstrak | 312.33 kB | Adobe PDF | View/Open |
2012asy_BAB I Pendahuluan.pdf Restricted Access | BAB I | 309.38 kB | Adobe PDF | View/Open |
2012asy_BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 344.66 kB | Adobe PDF | View/Open |
2012asy_BAB III Bahan dan Metode.pdf Restricted Access | BAB III | 366.71 kB | Adobe PDF | View/Open |
2012asy_BAB IV Hasill dan Pembahasan.pdf Restricted Access | BAB IV | 710.13 kB | Adobe PDF | View/Open |
2012asy_BAB V Simpulan dan Saran.pdf Restricted Access | BAB V | 291.41 kB | Adobe PDF | View/Open |
2012asy_Cover.pdf Restricted Access | Cover | 314.6 kB | Adobe PDF | View/Open |
2012asy_Daftar Pustaka.pdf Restricted Access | daftar pustaka | 349.21 kB | Adobe PDF | View/Open |
2012asy_Lampiran.pdf Restricted Access | Lampiran | 765.85 kB | Adobe PDF | View/Open |
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