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Title: | Characteristics and in vitro fertilization ability of cauda epididymal and ejaculated ram sperm Karakteristik dan Kemampuan Fertilisasi In Vitro Spermatozoa Domba yang Berasal dari Kauda Epididimis dan Ejakulat |
Authors: | Setiadi, Mohamad Agus Karja, Ni Wayan Kurniani Pamungkas, Fitra Aji |
Keywords: | spermatozoa cauda epididymal ejaculate in vitro fertilization ram |
Issue Date: | 2012 |
Abstract: | The characteristic and in vitro fertilization ability of ram spermatozoa collected from cauda epididymides was axamined. Ejaculated spermatozoa was used as control group in this experiment. Characteristic of spermatozoa including of percentage of progressive motility, viability, abnormalities and membrane integrity were evaluated before and after freezing. Fertilization ability of postthawed of the spermatozoa in both group was examined based on the pronucleus formation after in vitro fertilization of in vitro matured (IVM) oocytes. Results from these study showed that there were no significant differences in the characteristics between cauda epididymal and ejaculated spermatozoa before freezing (P>0.05). After freezing, motility of ejaculated spermatozoa was higher than epididymal spermatozoa (54.00±2.24% vs 48.00±4.47%), however the membrane integrity of epididymal spermatozoa was higher than ejaculated spermatozoa (75.38±9.32% vs 65.54±11.88%) (P<0.05). In vitro fertilization experiment revealed that the ability of post-thawed epididymal spermatozoa to fertilize oocytes (61.40%, 42.98%, 18.42% for total, normal and polysperm, respectively) did not differ from that of ejaculated spermatozoa (66.67%, 48.78%, 17.89% for total, normal and polysperm, respectively) (P>0.05). These results indicate that ram spermatozoa collected from cauda epididymal and then frozen have the ability to fertilize ram ooctyes in vitro in the similar rate with ejaculated spermatozoa. The new cryopreservation protocol and reproductive technology describe here can enhance conservation of ram genetic resources. |
URI: | http://repository.ipb.ac.id/handle/123456789/57301 |
Appears in Collections: | MT - Veterinary Science |
Files in This Item:
File | Description | Size | Format | |
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2012fap.pdf Restricted Access | Fulltext | 782.84 kB | Adobe PDF | View/Open |
Abstract.pdf Restricted Access | Abstract | 337.84 kB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 328.93 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 373.69 kB | Adobe PDF | View/Open |
BAB III Bahan dan Metode.pdf Restricted Access | BAB III | 382.42 kB | Adobe PDF | View/Open |
BAB IV Hasil dan Pembahasan.pdf Restricted Access | BAB IV | 532.44 kB | Adobe PDF | View/Open |
BAB V Kesimpulan.pdf Restricted Access | BAB V | 320.11 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 297.55 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 375.9 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 327.67 kB | Adobe PDF | View/Open |
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