Efikasi Bakteri Endofit dan Plant Growth Promoting Rhizobacteria dalam Menekan Perkembangan Penyakit Layu Bakteri (Ralstonia solanacearum) pada Tomat

Abstract

Tomato (Lycopersicum esculentum Mill.) is a popular vegetable in Indonesia, but the production levels are still fluctuated. One of the factors affected productivity is the incidence of bacterial wilt disease caused by Ralstonia solanacearum. Effective control to manage the disease which is environmentally friendly is the use of biocontrol agents. Application of endophytic bacterial (Staphylococcus epidermidis BC4) and Plant Growth Promoting Rizhobacteria (Pseudomonas fluorescens RH4003 and Bacillus subtilis AB89) as biocontrol of tomato bacterial wilt was expected to be an alternative tool. The objective of this study is to determine the efficacy of combination among endophytic bacteria and PGPR to suppress the development of bacterial wilt disease (R. solanacearum) and promote the growth of tomato. Depend on the growth test (growth rate and dry weight plant), the combination of bacteria that could promote the growth of tomato plant was combination of S. epidermidis BC4 75% with P.fluorescens RH4003 25%. That combination gave a better effect than the other treatments and control, but did not provide better suppression than treatment of single bacterium. Based on sinergism test, the relationship between PGPR and endophytic bacteria was an antagonistic. However, there were combination treatments which were able to suppress the disease, ie. combination of S. epidermidis BC4 50% with P. fluorescens RH4003 50% and the combination of S. epidermidis BC4 50% with B. subtilis AB89 50%.