Pengaruh Pemberian Ekstrak Minyak Jintan Hitam (Nigella sativa) Terhadap Organ Limfoid Sekunder Mencit (Mus musculus)

Abstract

Concerning to the people who were realized the importance of returning to a healthy lifestyle and back to nature was the reason of this research done using black seed (Nigella sativa). The purpose of this research was to study the effect of black seed oil extracts in secondary lymphoid organ of mice (Mus musculus). Twenty four of 4 weeks age mice were divided into 2 groups by gender. First group was male group and the other was female group. Both male and female groups were subdivided into 4 groups, each group consisted of three mice. Group I was control (received aquadest 0,1 ml), Group II (received 0,1 ml black seed oil Extracts), Group III (received 0,2 ml black seed oil extracts), and Group IV (received 0,3 ml combination of black seed oil Extracts and honey). This treatment were done for two months, the mice were then euthanized. The lymph node and spleen were collected as histopathological samples. Samples were processed routinely to prepare histopathology slide stained with Haematoxylin Eosin. The parameters observed were the number and width of lymphoid follicle on both spleen and lymph node. Quantitative data were analyzed with ANOVA test and followed by Duncan test. The observation results of that the mice given black seed oil extracts showed increased of lymphoid follicle wide of spleen and lymph node on group II, III, and IV which are significant (p<0,05) compare to the control group.