Deteksi Secara Imunohistokimia Imunoglobulin A (IgA) pada Usus Halus Tikus yang Diberi Bakteri Asam Laktat (BAL) dan Enteropatogenik Escherichia coli (EPEC)

Abstract

This study was conducted to evaluate the effect of Lactobacillus plantarum and Lactobacillus fermentum on the immunohistochemical profile of immunoglobulin A (IgA) and the intestinal mucosa thickness of Enteropathogenic E. coli (EPEC) treated rats. A total of 90 male Sparague Dawley rats were used in this study. They were divided into 6 groups: (1) negative control treated group, (2) Lactobacillus plantarum treated group, (3) Lactobacillus fermentum treated group, (4) Lactobacillus plantarum and EPEC treated group, (5) Lactobacillus fermentum and EPEC treated group, and (6) EPEC treated (positive control) group. The results showed that both Lactobacillus fermentum and Lactobacillus plantarum treatment for 2 to 3 weeks increased the thickness of rat’s mucosal small intestine. In the EPEC treated rats, either Lactobacillus fermentum and Lactobacillus plantarum treatment for 2 weeks increased the thickness of rat’s mucosal small intestine. Lactobacillus fermentum treatment for 2 weeks increased the content of IgA in rat’s mucosal small intestine at both conditions; with and without EPEC treatment. Lactobacillus plantarum treatment for 2 weeks only increased the content of IgA in rat’s duodenum and jejunum, while in the EPEC treated rats, Lactobacillus plantarum treatment for 2 weeks could maintained the content of IgA in rat’s mucosal small intestine. Lactobacillus fermentum gave better effect than Lactobacillus plantarum on increasing the content of IgA in rat’s small intestine