Primary Genetic Map Of A Wide Faba Bean (Vicia faba L.) Cross "Germany X Moroccan"

Abstract

This research was conducted at the Molecular Genetic Laboratory of Plant Breeding Department, Georg-August University, Germany, from 1999-2001. The main objective of this research is to develop a genetic linkage map in faba bean (Vicia faba L.). A Population of F7 recombinat inbred lines (RILs)derived from the cross 34 Marocco x Kristall 25 was used. The 34 Marocco is a pure line bred from ICARDA, Syria. Kristall 25, developed at Hohenheim, Germany, is an inbred line from German cv. Kristall. The RIL population consist of 253 lines. The number of lines analyzed with RAPD was 57. Those lines were chosen randomly from the whole population of RILS. Mapmaker /Exp. 3.0 was applied to the data. The Haldane function LOD > 3.0 and maximum like hood distance of 40 cM was used. The 48 selected decamer primers amplified a total of 115 polymorphic bands with an average of 2.3 polymorphic bands per primer. Fragment sizes ranged from about 550 bp to 2400 bp. The present map comparies a total of 858.3 cM and classifled into 14 linkage groups.