Aktivitas Antioksidan dan Sitotoksisitas Metabolit Sekunder Daun Salam (Syzygium polyanthum Wight.) dan Daun Jati Belanda (Guazuma ulmifolia Lamk.)

Abstract

Secondary metabolites from salam (Syzygium polyanthum Wight.) and jati belanda (Guazuma ulmifolia Lamk.) leaves can potentially be optimized for the utilization of these leaves as antioxidants. This research aimed to compare antioxidant activity and cytotoxicity of crude extracts of flavonoid, tanin, and hydroquinone of both type of leaves. Total phenolic and flavonoid compound was measured trough colorimetry, then the antioxidant activity was measured using 1.1-diphenil-2-picrylhydrazyl (DPPH) method and cytotoxicity test use brine shrimp lethality test (BSLT). Total phenolic value of salam leaves (614.70 mg GAE/100 g dried leaves) was higher when compared to jati belanda leaves (566.824 mg GAE/100 g dried leaves). Similarly, total flavonoids of salam leaves (434.234 mg QE/100 g dried leaves) was higher when compared to jati belanda leaves (246.214 mg QE/100 g dried leaves). This value is supported by IC50 values with DPPH method and LC50 values with cytotoxicity test showed the lowest IC50 and LC50 is flavonoid extract of salam leaves (IC50 = 10.833 μg/mL and LC50 = 328.427 μg/mL). Flavonoid of Salam leaves is the secondary metabolites with the best antioxidant activity and bioactivity effect.