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Title: | Pemurnian dan karakterisasi xilanase ekstraseluler dari streptomyces sp. 234P-16 asal Padang FAHRURROZI. Purification and Characterization of Extracellular Xylanase from Streptomyces sp. 234P 16 Isolated from Padang. |
Authors: | Meryandini, Anja Ahkdiya, Alina Fahrurrozi |
Keywords: | Bogor Agricultural University (IPB) Xylanase Purification Characterization |
Issue Date: | 2007 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Xylan is the major component of plant hemicellulose and the second most abundant polysaccharide in nature. As a renewable substance, xilan can be degraded as useful end product. Xylanases is a group of enzyme that has ability to hydrolyse hemicellulose into xylan or polymer of xyloses and @ xylooligosaccharides. Complete hydrolysis of xylans involves the synergistic action ofaxylanolytic enzymes, including 13.-xylanase, f!,-xylosidase, :::I: a-glucuronidase, a-L-arabinofuranosidase, and acetyl xylan esterase. ~ Streptomyces sp. 234P-16 produced extracellular xylanase that had unique o characteristics: optimum temperature and pH was 90°C and 5.0 respectively. '2. The purpose of this research was to purify and characterize' the xylanase of III 3 Streptomyces sp. 234P-16. Streptomyces sp. 234P-16 was able to produce two _. xylanolytic enzymes a-L-arabinofuranosidase and (3-xilosidase. Crude extract of '" xylanase was precipitated by 60-70% acetone gradually, was purified with anion ~ exchange chromatography using Sephadex A-50 and gel filtration chromatography using Sephadex G-100. The molecular mass of the purified ~ xylanase after ion-exchange was 42.456 kDa and 16.903 kDa but gel filtration ~ showed the molecular mass 42.456 kDa. The optimum temperature and pH was S. 90°C and 5.0 respectively. The xylanase could resist of heating at 90°C for ~ 180 minute. The result of kinetic analysiS showed that Vmax was 523 :l IJmol/minute/mg and Km was 1.98 mg/ml. Xilan adalah komponen terbesar hemiselulosa tanaman dan merupakan polisakarida terbesar kedua setelah selulosa. Xilan mempunyai potensi yang sangat besar untuk diuraikan menjadi produk akhir yang berguna. Xilanase ~upakan kelompok enzim yang menghidrolisis hemiselulosa dalam hal ini ialah ~n atau polimer dari xilosa dan xilooligosakarida. Pemecahan sempuma xilan nfmerlukan aktivitas sinergis beberapa enzim hidrolitik (hemiselulase), yaitu ePrrlo-1 ,4-r1-xilanase, ~-xilosidase, a-glukuronidase, a-L-arabinofuranosidase, ~n asetil xilan esterase. Streptomyces sp. 234P-16 menghasilkan xilanase etl!;traseluler dengan sifat yang unik yaitu mempunyai suhu optimum 90°C dan pel optimum 5,0. Penelitian ini bertujuan untuk melakukan pemurnian serta kiakterisasi biokimiawi xilanase ekstraseluler yang dihasilkan oleh StrPptomyces sp. 234P-16. Streptomyces sp. 234P-16 dapat memproduksi dua e~im xilanolitik yaitu a-L-arabinofuranosidase dan (3-xilosidase. Xilanase ekstrak kiiar diendapkan dengan 60-70% aseton secara bertahap, dimurnikan dengan k~m atografi penukar anion Sephadex A-50 dan terakhir dimurnikan ~nggunakan kromatografi filtrasi gel Sephadex G-100. Berat molekul xilanase setelah dimumikan dengan penukar anion, yaitu 42,456 dan 16,903 kDa, namun sE!elah dimurnikan dengan filtrasi gel 42,456 kDa. Suhu dan pH optimum 90°C dci1 5,0. Stabil pada suhu 90°C selama 180 menit. Pengukuran terhadap p:!ameter kinetika menghasilkan Vmaks = 523 ~mol/menitlmg dan KIOJ= 1,98 mg/ml |
URI: | http://repository.ipb.ac.id/handle/123456789/55193 |
Appears in Collections: | MT - Agriculture |
Files in This Item:
File | Description | Size | Format | |
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2007fah.pdf Restricted Access | full text | 2.71 MB | Adobe PDF | View/Open |
Abstract.pdf Restricted Access | Abstract | 332.81 kB | Adobe PDF | View/Open |
BAB I Pendahuluan.pdf Restricted Access | BAB I | 395.83 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka.pdf Restricted Access | BAB II | 754.48 kB | Adobe PDF | View/Open |
BAB III Bahan dan Metode.pdf Restricted Access | BAB III | 506.54 kB | Adobe PDF | View/Open |
BAB IV Hasil dan Pembahasan.pdf Restricted Access | BAB IV | 959.81 kB | Adobe PDF | View/Open |
BAB V Kesimpulan dan Saran.pdf Restricted Access | BAB V | 297.75 kB | Adobe PDF | View/Open |
Cover.pdf Restricted Access | Cover | 281.61 kB | Adobe PDF | View/Open |
Daftar Pustaka.pdf Restricted Access | Daftar Pustaka | 541.4 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 610.9 kB | Adobe PDF | View/Open |
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