White blood cell response after implantation of wild natural silk (Attacus atlas L) as a biomaterial surgical suture

Abstract

White blood cell response after implantation of wild natural silk (Attacus atlas L.) as a biomaterial surgical suture was described in this study. Twenty four 8-10 weeks old of male mices were used in this study. They were divided into 4 groups: control, commercial silk, plain cut gut, and natural silk of Attacus atlas L.. Each groups except control was implanted with different suture materials within 1,5cm length wound on the right femoral muscle. The blood were collected from blood venous at sinus retroorbitalis of anesthetized mices by using microhaematocrit tube on first day before and 7 day post after implantation. The result showed that the number of white blood cell before implanted are normally (9.2±1.3 x 103 cells) but after 7 day implanted in natural silk Attacus atlas L. group (11.3±1.4 x 103 cells) were higher compared with control (8.0±0.5 x 103 cells), but slightly higher than commercial silk (10.9±0.6 x 103cells), and lower than catgut (14.1±1.2 x 103 cells) as highed response sample group. This result indicated that Attacus atlas L. silk had same performed with the commercial silk suture.